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5% v/v caused lysis of phosphatidylcholine vesicles during repeated cycling through phase transition temperatures. 41 Partitioning of ethanol into the membrane can be altered through the presence of additional biologically relevant lipid species such as cholesterol or gangliosides. 43,60,61 This indicates that complex biological membranes may respond very differently, and not only the presence of other lipid types, but also the impact of proteins need to be taken into account. Nonetheless, it is clear that model membranes are sensitive to ethanol at concentrations far lower than the 60%70% currently recommended for inactivation of virus on hard surfaces, and at amounts contained in widely available mouthwashes Section 6. We also reviewed the impact of ethanol on mammalian cells in vitro. Of direct relevance to coronaviruses, a study on corneal epithelial cells showed that a 30 s incubation with 20% ethanol led to around 40% loss of viability, which increased to 70% loss at 40% ethanol. There was significant leakage of intracellular contents following 20% ethanol for 30 s. 48 This short incubation also altered inflammatory responses, differentiation, and epithelial marker expression. 48 Several studies on the impact of ethanol on red blood cells were also found. Sonmez et al. showed that around 1 M 5.

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